Journal: Drug Design, Development and Therapy

Article Title: PJ34 Prevents Trauma-Induced Heterotopic Ossification without Adverse Bone Healing: An in vivo and in vitro Investigation

doi: 10.2147/DDDT.S561571

Figure Lengend Snippet: Cytotoxicity and cell viability with PJ34 in vitro. The effect of PJ34 on BMSCs cytotoxicity at 12 and 24 h ( a ). The effect of PJ34 on BMSCs proliferation at 1, 4, 7 days ( b ). Live (green) /dead (red) staining of BMSCs at 1, 4, 7 days ( c ). Cell morphology of BMSCs at 1, 4, 7 days ( d ). The cytoplasm of BMSCs was stained with phalloidin (d, red), and cell nucleus were stained with DAPI (d, blue) to further observe the cell morphology. n=5 per group. *p < 0.05.

Article Snippet: PJ34 were purchased from MedChemExpress (MCE, USA).

Techniques: In Vitro, Staining

Journal: Drug Design, Development and Therapy

Article Title: PJ34 Prevents Trauma-Induced Heterotopic Ossification without Adverse Bone Healing: An in vivo and in vitro Investigation

doi: 10.2147/DDDT.S561571

Figure Lengend Snippet: Biological function of PJ34 in vitro. Migration of HUVECs in a scratch wound healing assay in vitro at 0 and 24 h after treated with PJ34 ( a ). Transwell migration assay of BMSCs ( b ). Percentage of wound closure determined by the microscopic photographs ( c ). Quantitative analysis of the number of BMSC cells passing through the chamber (per field) ( d ). n=5 per group. *p < 0.05.

Article Snippet: PJ34 were purchased from MedChemExpress (MCE, USA).

Techniques: In Vitro, Migration, Wound Healing Assay, Transwell Migration Assay

Journal: Drug Design, Development and Therapy

Article Title: PJ34 Prevents Trauma-Induced Heterotopic Ossification without Adverse Bone Healing: An in vivo and in vitro Investigation

doi: 10.2147/DDDT.S561571

Figure Lengend Snippet: Osteogenesis-inducing capacity of PJ34 in vitro. The ALP activity of BMSCs for 7 and 14 days ( a ). ARS staining of BMSCs for 14 and 21 days ( b ). Quantitative analysis of the ALP activity of BMSCs ( c ). Quantitative analysis of the OD value for ARS staining of BMSCs ( d ). Dotted circles represent the gross images of each well in 12-well plate. n=5 per group. *p < 0.05.

Article Snippet: PJ34 were purchased from MedChemExpress (MCE, USA).

Techniques: In Vitro, Activity Assay, Staining

Journal: Drug Design, Development and Therapy

Article Title: PJ34 Prevents Trauma-Induced Heterotopic Ossification without Adverse Bone Healing: An in vivo and in vitro Investigation

doi: 10.2147/DDDT.S561571

Figure Lengend Snippet: Evaluation of osteogenic potential of PJ34 under different concentrations using qRT-PCR and Western blot analysis in vitro. Effect of the osteogenic medium with PJ34 on the osteogenic gene expression in BMSCs at 3 days, including Col I ( a ), Runx2 ( b ), BMP2 ( c ), ALP ( d ), BGLAP ( e ). Verification of PARP-1 inhibition by PJ34 ( f and g ). Effect of the osteogenic medium with PJ34 on the osteogenic protein expression in BMSCs at 3 days ( f – h ), including Col III ( i ), Col I ( j ), OPN ( k ), Runx2 ( l ). n=5 per group. *p < 0.05.

Article Snippet: PJ34 were purchased from MedChemExpress (MCE, USA).

Techniques: Quantitative RT-PCR, Western Blot, In Vitro, Gene Expression, Inhibition, Expressing

Journal: Drug Design, Development and Therapy

Article Title: PJ34 Prevents Trauma-Induced Heterotopic Ossification without Adverse Bone Healing: An in vivo and in vitro Investigation

doi: 10.2147/DDDT.S561571

Figure Lengend Snippet: Evaluation inhibition of HO by PJ34 in vivo. Micro-CT of HO in control groups ( a ) and PJ34 groups ( b ) at 8 weeks. Quantitative analysis of BV at 8 weeks ( c ). Representative photographs of HE-stained section of control and PJ34 groups at 8 weeks ( d ). Red dotted squares indicating distal area of Achilles tendon transection, yellow dotted squares indicating proximal area of Achilles tendon transection. Red arrows indicating HO. n=6 per group. *p < 0.05.

Article Snippet: PJ34 were purchased from MedChemExpress (MCE, USA).

Techniques: Inhibition, In Vivo, Micro-CT, Control, Staining

Journal: Drug Design, Development and Therapy

Article Title: PJ34 Prevents Trauma-Induced Heterotopic Ossification without Adverse Bone Healing: An in vivo and in vitro Investigation

doi: 10.2147/DDDT.S561571

Figure Lengend Snippet: Effect of PJ34 on fracture healing. Photographs showing tibia fracture process in a mouse model ( a ). Micro-CT and X-ray showing healing process of tibia fracture in control group ( b ) and PJ34 group ( c ) at 1, 2, 3, and 4 weeks. BV analysis determined by micro-CT at 1, 2, 3, and 4 weeks ( d – g ). n=5 per time.

Article Snippet: PJ34 were purchased from MedChemExpress (MCE, USA).

Techniques: Micro-CT, Control

Journal: Drug Design, Development and Therapy

Article Title: PJ34 Prevents Trauma-Induced Heterotopic Ossification without Adverse Bone Healing: An in vivo and in vitro Investigation

doi: 10.2147/DDDT.S561571

Figure Lengend Snippet: Effect of PJ34 on fracture healing. Histological observation of tibia fracture healing process by HE staining in control group ( a ) and PJ34 group ( b ) at 1, 2, 3, and 4 weeks. Red dotted squares indicating top side of tibial fracture healing area, yellow dotted squares indicating bottom side of tibial fracture healing area. n=5 per time.

Article Snippet: PJ34 were purchased from MedChemExpress (MCE, USA).

Techniques: Staining, Control